Functional programming of membrane-bound receptors by On-cell Coordination Chemistry

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06318
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bio-related chemistry
• Research Institution: Kyoto University
• Principal Investigator: Kubota Ryou 京都大学, 工学研究科, 助教 (00753146)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 金属錯体 / 化学遺伝学 / GPCR / リガンド作動性イオンチャネル / 遺伝子工学

Outline of Final Research Achievements

Membrane-bound receptors in living cells mediate signal transductions by receiving extracellular signals. Despite their biological importance, it is still quite difficult to unveil their functions due to their similar and complicated three-dimensional structures and activation mechanisms. Here we have developed novel chemogenetic approaches, called On-cell Coordination Chemistry (OcCC) in order to artificially control the functions of membrane-bound receptors. We successfully demonstrated that OcCC enabled to control the activities of two types of neurotransmitter receptors, ionchannel type and GPCR type glutamate receptors, in live neurons. Also, we achieved the artificial activation of class A GPCRs by coordination anchor method.

Free Research Field

錯体化学、超分子化学、ケミカルバイオロジー