Studies on acyltransferases that create the heterogeneity of biological membrane in bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H06328
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Ogawa Takuya 京都大学, 化学研究所, 助教 (40756318)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: リゾホスファチジン酸アシル基転移酵素 / 低温適応 / 脂質代謝

Outline of Final Research Achievements

I analyzed the functions of five homologs (SlPlsC1-SlPlsC5) of 1-acyl-sn-glycerol-3-phosphate acyltransferase (PlsC) from the psychrotrophic bacterium Shewanella livingstonensis Ac10. 1) I established the methods of purification and enzymatic assay for SlPlsC1 and revealed its enzymatic characteristics in vitro. 2) It was indicated that SlPlsC3 is involved in the biosynthesis of a yellow pigment of which the chemical structure is as yet unidentified, suggesting a novel function of PlsC-family enzymes. In addition, 3) aiming at the crystal structure analysis of PlsC, I purified a thermostable PlsC from the thermophilic bacterium Thermus thermophilus and revealed its enzymatic characteristics.

Free Research Field

酵素、微生物