2016 Fiscal Year Final Research Report
The effect of skin microbiome from atopic dermatitis on skin immunity
| Project/Area Number |
15H06428
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | アトピー性皮膚炎 / ランゲルハンス細胞 / 黄色ブドウ球菌 |
|---|
| Outline of Final Research Achievements |
The skin of patients with atopic dermatitis (AD) presents as a disbalance of the microbiome with a strong colonization by Staphylococcus aureus. However, the effect of colonized S. aureus on the skin immune system has not been fully elucidated. We explored whether S. aureus TF3378 isolated from AD skin was able to skew T cell responses via Langerhans cells (LC) as compared to a standard strain of S. aureus NCTC8325. Monocyte-derived LC (MoLC) from healthy controls and patients with AD were stimulated with S. aureus NCTC8325 or TF3378. S. aureus-stimulated MoLC were co-cultured with autologous CD4pos T cells and then T cell responses were analyzed. MoLC stimulated by S. aureus TF3378 induced significantly high and rapid proliferation of T cells with less Th1 cytokine (IFN-γ) production. The mRNA from T cells after co-culture showed imbalanced Th1/Th2 expression. These data illustrate S. aureus TF3378 on AD skin can skew T cell responses via LC toward imbalanced Th1 / Th2 skin immunity.
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| Free Research Field |
皮膚科
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