In vivo two photon imaging of visual top-down connection in primates

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06473
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo (2016); Kyushu University (2015)
• Principal Investigator: Matsui Teppei 東京大学, 大学院医学系研究科(医学部), 助教 (10725948)
• Research Collaborator: MURAKAMI Tomonari ; HASHIMOTO Takayuki ; UEMURA Masato
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 視覚 / 二光子 / カルシウムイメージング

Outline of Final Research Achievements

There were two technical challenges that need to be overcome. First goal was to establish a method to express GCaMP in marmoset cortical neurons at a level sufficient for in vivo imaging. We accomplished this goal by finding an appropriate promoter for driving GCaMP expression in marmoset neurons. Second goal was to have columner level functional mapping over marmoset visual cortex. We confirmed that traditional intrinsic signal optical imaging can meet this goal. We further established a wide-field calcium imaging technique that far exceed the intrinsic signal imaging in both temporal resolution and signal to noise ratio. Combination of these two techniques will enable functional mapping of top down signal in the marmoset visual cortex.

Free Research Field

神経科学