Epidemiological and molecular analysis of decreased susceptibility to fluoroquinolone in Haemophilus influenzae

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06521
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including mycology)
• Research Institution: Sapporo Medical University
• Principal Investigator: SATO TOYOTAKA 札幌医科大学, 医学部, 助教 (30756474)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: フルオロキノロン耐性 / インフルエンザ菌

Outline of Final Research Achievements

Haemophilus influenzae is present as a commensal organism in upper respiratory tract and cause respiratory tract infections. Fluoroquinolone is one of the potent antimicrobial agent for the treatment. Although detail mechanical analysis of fluoroquinolone resistant development is important for prevent the emergence and spreading of fluoroquinolone resistant H. influenzae, that mechanism has been unclear. In this study, all H. influenzae clinical isolates exhibited susceptible to fluoroquinolone. However, 2 of the 64 (3.1%) isolates decreased susceptibility to fluoroquinolone with an amino acid substitution in QRDR. in vitro fluoroquinolone exposer experiment showed that 55.2% of H. influenzae clinical isolates decreased fluoroquinolone susceptibility and 34.4% was developed fluoroquinolone resistance. We also observed the significant association between decreased susceptibility to fluoroquinolone and ampicillin resistance.

Free Research Field

細菌学