Functional analysis of bone morphogenetic protein receptor II

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06568
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Saitama Medical University
• Principal Investigator: OHTE Satoshi 埼玉医科大学, 医学部, 助教 (00547979)
• Research Collaborator: KATAGIRI Takenobu 埼玉医科大学, 医学部, 教授 ; TSUKAMOTO Sho 埼玉医科大学, 医学部, 助手 ; KURATANI Mai 埼玉医科大学, 医学部, 研究員
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 骨形成因子 / PAH

Outline of Final Research Achievements

Bone morphogenetic proteins (BMPs) are cytokines, which important for bone development. Intracellular BMP signaling is activated by type I and type II BMP receptors. These receptors contain intracellular kinase domain, which consist of about 300 amino acids. A long form (LF) of BMPR-II has a unique 500 amino acid tail domain at the C-termini. Several mutations in the tail domain of BMPR-II have been identified in patients with pulmonary arterial hypertension, suggesting that the tail domain has a novel function in BMP signaling. In this study, we analyzed a function of the BMPR-II tail domain. Western blot analysis revealed that protein level of BMPR-II LF was lower than that of BMPR-II short form (SF), but mRNA level seemed comparable. BMPR-II SF induced higher ALP activity than that of BMPR-II LF in C2C12 cells co-expressed with a BMP type I receptor. These findings suggest that BMPR-II LF is suppressed the activity by the tail domain through a reduction of protein stability.

Free Research Field

病態生理学