Elucidation of the mode of action of kurahyne, an ER-stress inducer

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06584
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biomolecular chemistry
• Research Institution: Keio University
• Principal Investigator: Iwasaki Arihiro 慶應義塾大学, 理工学部(矢上), 助教 (00754897)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: クラハイン / 海洋天然物化学 / SERCA / プロヒビチン / 破骨細胞

Outline of Final Research Achievements

Kurahyne is a marine natural product that exhibits osteoclast differentiation-inhibitory activity and sarco/endoplasmic reticulum Ca2+-ATPase (SERCA)-inhibitory activity. In this study, I have investigated the detailed mechanisms of these activities.
As a result, we have found the following things;
(1) Prohibitin-1 and -2 can play an important role in the osteoclast differentiation-inhibitory activity of kurahyne; (2) Specific fourteen amino-acid residues in SERCA are identified as a possible kurahyne-binding site; (3) Two kurahyne-related marine natural products were isolated, and the structure determination and the total synthesis of them were achieved. Furthermore, some knowledge regarding a structure-activity relationship was obtained.

Free Research Field

天然物化学