2016 Fiscal Year Final Research Report
Development of neuron-specific analytical method to elucidate the mechanism underlying AD pathogenesis
| Project/Area Number |
15H06587
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Keio University |
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Principal Investigator |
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| Research Collaborator |
Shen Jie ハーバード大学, 医学部, 教授
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | アルツハイマー病 / シングルセル / 神経変性疾患 / モデルマウス |
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| Outline of Final Research Achievements |
In this research proposal, I aimed to isolate single neuron from entorhinal cortex layer 2/3 of adult mouse, which is most likely vulnerable neuronal population in Alzheimer’s disease patients. First, I dissected out entorhinal cortex layer 2/3 from mouse brain slice under stereoscopic microscope, followed by trypsin treatment. Next, I picked single neurons with micropipette, and cDNAs fom each single neuron were synthesized for RT-PCR analysis. Several marker genes specific for entorhinal cortex layer 2/3 can be successfully detected by semiquantitative RT-PCR.
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| Free Research Field |
神経科学
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