2016 Fiscal Year Final Research Report
Study of IEC-IEL interraction in the development of gut epithelial immune system.
| Project/Area Number |
15H06589
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Keio University |
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Principal Investigator |
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| Research Collaborator |
Kronenberg Mitch La Jolla Institute for Allergy & Immunology, Division of Developmental Immunology, President & Chief Scientific Officer
Seo Goo-young La Jolla Institute for Allergy & Immunology, Division of Developmental Immunology, Postdoctoral fellow
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | 上皮細胞 / 上皮細胞間Tリンパ球 |
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| Outline of Final Research Achievements |
Ap1m2 is predominantly expressed by intestinal epithelial cells (IECs) and functions in the polarized transport of membrane proteins to the basolateral membrane of IECs. We have found the lack of CD8aa expressing intestinal intraepithelial lymphocytes (IELs) in Villin-creERT2 Ap1m2 flox/flox mice when treated with tamoxifen. CD8aa IELs of the IEC specific Ap1m2-deficient mice showed higher levels of Bcl-2 and Bcl-xL expression compared to control littermate mice. These cells also exhibited higher proliferations. These data suggest that protein localization of basolateral membrane on IEC is indispensable for CD8aa IELs numbers and phenotypes. We have established a monolayer culture method with crypts isolated from Villin-creERT2 Ap1m2 flox/flox mice. This monolayer formed tight junctions, and contains both apical and basolateral membrane domains, which is the important characteristic of polarized epithelial cells.
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| Free Research Field |
粘膜免疫学
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