The establishment of an universal gene targeting system with novel positive-negative selection in plants

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06853
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Science in genetics and breeding
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Nishizawa-Yokoi Ayako 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 遺伝子利用基盤研究領域, 研究員 (10760019)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: ジーンターゲッティング / ポジティブ・ネガティブ選抜 / キメラリプレッサー / ファイトスルフォカイン

Outline of Final Research Achievements

We designed novel conditional positive-negative selection system using the repression of positive selection marker via chimeric repressor (SRDX/nptII) for isolation of GT cells. The expression of SRDX led to decreased number of kanamycin-resistant callus from transgenic tobacco leaf under the selection conditions, suggesting that this approach would be able to be employed for the positive-negative selection of GT cells. In addition, we tested the effect of the plant peptide growth factor, phytosulfokine, on cell growth of transgenic rice calli. There was no significant difference in cell growth between phytosulfokine-expressing transgenic calli and control calli. We are trying to establish GT system with SRDX/nptII system as a positive-negative selection in tobacco.

Free Research Field

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