Development of the catalog of various exosomes for efficient drug delivery by the analysis of their physicochemical property-pharmacokinetics relationship

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06865
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Warashina Shota 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 特別研究員 (30755393)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: エクソソーム / DDS

Outline of Final Research Achievements

The purposes of the research are (1) to show the usefulness of making exosome catalog by the analysis of exosome physicochemical property-pharmacokinetics relationship and (2) to establish drug incorporation method into exosomes without character changes for the development of exosome DDS. Through (1), we have established the evaluation method of exosome physicochemical property and pharmacokinetics (including cellular uptake). Especially, we succeeded in the potential of affinity difference against cancer cells between some types of exosomes. Moreover, we developed labeling method of positron emitter onto exosomes for pharmacokinetics analysis by PET imaging. In (2), we have developed the method of siRNA incorporation into exosomes by liposome-based membrane fusion without size changes. Using these techniques, we are going to evaluate the pharmacokinetics of the exosomes in mice, analyze the relationship and make a catalog for targeting cancer.

Free Research Field

ドラッグデリバリー