Development of Quality Control Method in Regenerative Medicine Products Using a Bioradiography

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01297
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kitasato University
• Principal Investigator: Sasaki Toru 北里大学, 医療衛生学部, 准教授 (30158927)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 還元ストレス / 活性酸素 / レドックス / 酸化ストレス / 再生医療 / 分子イメージング / 品質管理

Outline of Final Research Achievements

We investigated reactive oxygen species (ROS) generation in cerebral cortex slices during the restoration of hypoxia caused by a decreased oxygen supply and a transiently enhanced energy metabolism, with tissue pO2 changes and redox balance using real-time bioradiography. ROS generation was transiently enhanced during reoxygenation in both hypoxic cases. The redox balance shift towards reduction “hyper-reduction” in tissue were suggested to participate in increased ROS generation under the restoration of transient hypoxia induced by a decreased oxygen supply and increased oxygen demand. To investigate whether it is possible to estimate the safety, soundness and effectiveness of transplantable tissues and organs by FDG-bioradiography method in a non-invasive manner, we examined the FDG uptake in reconstructed human cultured epidermal model. This result suggested the possibility of quality control in regenerative medical products using FDG - biodiography.

Free Research Field

放射線科学