Development of Novel Antitumor Treatment by Sonodynamically Activated Cancer-oriented Carbon Nanotubes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01343
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Yokohama College of Pharmacy
• Principal Investigator: Shigeyama Masato 横浜薬科大学, 薬学部, 教授 (90598327)
• Co-Investigator(Kenkyū-buntansha): 岩瀬 由未子 横浜薬科大学, 薬学部, 講師 (00521882) ; 梅村 晋一郎 東北大学, 医工学研究科, 教授 (20402787) ; 弓田 長彦 横浜薬科大学, 薬学部, 教授 (40191481)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 超音波 / がん指向性カーボンナノチューブ / アポトーシス / 音響化学療法 / ナノ粒子

Outline of Final Research Achievements

The present study aims to investigate sonodynamically-induced apoptosis using the Polyhydroxy Carbon Nanotube (PHCN). HL-60 cells were exposed to ultrasound in the absence and presence of PHCN. Apoptosis was analyzed by cell morphology and caspase-3 activity. The number of apoptotic cells showing membrane blebbing and cell shrinkage after combined treatment (ultrasound and PHCN) was significantly higher than following other treatments, including ultrasound alone and PHCN alone. Furthermore, Enhancement of caspase-3 activation was observed in cells treated with ultrasound and PHCN. Sonodynamically induced apoptosis and caspase-3 activation were significantly suppressed by histidine. These results indicate that the combination of ultrasound and PHCN induced apoptosis by reactive active oxygen in HL-60 cells.

Free Research Field

臨床薬剤学