2017 Fiscal Year Final Research Report
Relationship of the mitogen-activated protein kinase with skeletal muscle fiber size and the changes of fiber type composition
| Project/Area Number |
15K01620
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Sports science
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
Roy Roland R. University of California Los Angeles, Department of Integrative Biology and Physiology and Brain Research Institute
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | ラット骨格筋 / MAPK / ERK1/2 / 徐神経 / ヒラメ筋 / 足底筋 / 腓腹筋 / リン酸化 |
|---|
| Outline of Final Research Achievements |
The purpose of the present study is to clarify the muscle fiber phenotype specificity of 42/44 kDa MAPK (ERK1/2) protein. Expression level of phosphorylated ERK 1/2 was analyzed for the 10-wk-old Wistar rat and the level was hoghest in the superficial portion of the gastrocnemius muscle, lower in the plantaris and deep gastrocnemius, and lowest in the soleus muscle. This order is the same with the ratio of fast type fiber containing in each muscle. Rat soleus muscle contained the fast type fiber highest in the 3-wk-old, and gradually decrease during growing and aging stages. Similar change was observed in the expression level of phosphorylated ERK1/2. In addition, the increase in the fast-phenotype fiber and the phosphorylated ERK1/2 was observed in the denervated rat soleus muscle.
These results strongly suggest that the expression of phosphorylated ERK1/2 is closely related to that of the fast phenotype fiber in rat skeletal muscles.
|
| Free Research Field |
運動生理学、骨格筋生化学
|
