Elucidation of antiviral mechanism of sulfated polysaccharides

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05617
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Polymer/Textile materials
• Research Institution: Kitami Institute of Technology
• Principal Investigator: Yoshida Takashi 北見工業大学, 工学部, 教授 (40166955)
• Co-Investigator(Kenkyū-buntansha): 中島 秀喜 聖マリアンナ医科大学, 医学部, 教授 (20192669)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 硫酸化糖鎖 / HIV / 相互作用 / ペプチド

Outline of Final Research Achievements

Sulfated polysaccharides were found to have potent antiviral activity such as HIV, dengue fever, influenza viruses, assumed by an electrostatic interaction between negatively charged sulfated groups and positively charged surface glycoproteins of viruses. We have investigated the interaction mechanism of sulfated polysaccharides and a model compound of the proteins, polylysine, using surface plasmon resonance (SPR). In this work, we present a quantitative analysis of the interaction using peptides from HIV-1 surface glycoprotein gp120.
The association (ka) and dissociation-rate (kd) constants were ka=5.69×105 1/Ms, kd=2.25×10-4 1/s, and dissociation constant (KD) KD=3.95×10-10, respectively. The peptides were found to be interacted strongly with curdlan sulfate, however, the peptide C showed no interaction. Dextran sulfate gave the similar results.

Free Research Field

生体高分子