2017 Fiscal Year Final Research Report
Multi functionalization of proteins by sterically bulky caging
| Project/Area Number |
15K06575
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ケージド化合物 / タンパク質性医薬品 / コールドチェーン問題 / タンパク質の安定化 / PEGylation / タンパク質デリバリー / ナノ凝集体 / 超音波応答性 |
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| Outline of Final Research Achievements |
We created multi-functional caged proteins by modifying sterically bulky protection groups through stimuli-responsive cleavable linkers. Modification of a hydrophilic polymer as the protection group led to strongly stabilize the proteins, so that the modified protein could avoid aggregation even when they were heated. Furthermore, the modified protein was confirmed to drastically recover the activity via light-induced release of the protection group. This molecular technology is a promising tool for stabilizing protein drugs during their storage and transportation. In addition, protein aggregation through a sterically bulky protection group was serendipitously found, and the photolytic protein nano-aggregates were applied to protein regulation and delivery. Moreover, we obtained the preliminary results of both the reductant-responsive and ultrasonic-responsive linkers for expanding the responsibility of the present caged proteins to other external stimuli.
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| Free Research Field |
化学生物工学
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