Quiescent center-specific regulation of ribosome levels and elucidation of its developmental function

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Rikkyo University
• Principal Investigator: HORIGUCHI Gorou 立教大学, 理学部, 准教授 (70342847)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: シロイヌナズナ / 根端分裂組織 / 静止中心 / リボソームタンパク質 / RPL4D / ブラシノステロイド

Outline of Final Research Achievements

Quiescent center (QC) resides in the center of a root apical meristem and has a very low mitotic activity. To establish this quiescence, the amount of ribosomes is assumed to be maintained at a low level. In this study, overexpression of ribosomal protein fused with GFP revealed that at least several distinct ribosomal proteins are maintained at a low level in the QC cells. This regulation is counteracted by the exogenous application of brassinosteroids or cytokinins. In addition, such quantitative regulation of ribosomal proteins requires two mechanisms: one of them suppresses ribosome production in the QC cells and the other eliminates ribosomal proteins produced in excess in entire root apical meristem.

Free Research Field

植物発生学