Development of stealth-nanoparticles avoiding animal immune system that mimics the cell surface of filamentous fungi.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tohoku University
• Principal Investigator: Yoshimi Akira 東北大学, 未来科学技術共同研究センター, 准教授 (60436102)
• Co-Investigator(Renkei-kenkyūsha): ABE Keietsu 東北大学, 農学研究科, 教授 (50312624) ; KAWAKAMI Kazuyoshi 東北大学, 医学系研究科, 教授 (10253973) ; TAKAMI Seiichi 東北大学, 多元物質科学研究所, 准教授 (40311550)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 糸状菌 / 細胞壁 / ステルス素材 / α-1,3-グルカン / オリゴ糖合成 / 免疫細胞応答試験 / マウス免疫試験

Outline of Final Research Achievements

This study aims to create medical nanoparticles with stealth function using α - 1,3 - glucan (AG) and hydrophobin (HP) which are novel stealth materials derived from filamentous fungi. The results obtained in this study are as follows.
1) Determination of purification conditions for AG and AG oligosaccharides. 2) Confirmation of the stealth function of AG and AG oligosaccharides. 3) Confirmation of non-interaction between AG and HP. 4) Determination of enzymatic synthesis conditions of AG and AG oligosaccharides. 5) Demonstration of the stealth function of AG in vivo. 6) Evaluation of adsorption conditions of AG oligosaccharides and the particles.7) Discovery of galactosaminogalactan (GAG) as hyphal adhesion factor in Aspergillus oryzae. 8) Demonstration of additive interactions on the stealth function of AG and GAG.

Free Research Field

応用微生物学