Analysis and application of prophage-mediated gene reconstitution

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07371
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Hosei University
• Principal Investigator: SATO Tsutomu 法政大学, 生命科学部, 教授 (70215812)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: DNA組換え / 胞子形成 / 細菌 / 部位特異的組換え / 遺伝子再構築

Outline of Final Research Achievements

Bacillus subtilis SPβ prophage is excised from the host chromosome in a process of sporulation to reconstitute a composite spsM. As results of in vitro recombination experiments, we revealed that the site-specific recombination occurred at the position of AA in the center of recombination site “5'-ACAGATAA /AGCTGTAT-3 '” catalyzed by an integrase SprA. Furthermore, we showed that a recombination directionality factor (RDF) SprB is necessary for synaptic complex formation between SprA and substrate DNA during excision while SprB inhibits the formation during integration. We also developed a detection system for B. subtilis dedifferentiated cells from mother cells and new genome modifying tool based on the SPβ recombination function.

Free Research Field

ゲノム微生物