Molecular mechanism of amino acid signal transduction system in thermophilic bacterium

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Tomita Takeo 東京大学, 生物生産工学研究センター, 助教 (50447364)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: アミノ酸代謝調節 / アミノ酸シグナル / グルタミン酸脱水素酵素 / 高度好熱菌 / ロイシン / アロステリック調節 / タンパク質間相互作用 / 結晶構造解析

Outline of Final Research Achievements

We found that glutamate dehydrogenase (GDH) from Thermus thermophilus interacted with a homolog of adenine phosphoribosyltransferase (APRTh). The GDH/APRTh complex was subject to allosteric activation by leucine and AMP. So far, only GDHs from mammalian have been thought to be subject to complicated allosteric regulation. We, however, found that such a complicated regulation of GDH from the organism except for mammalian. This result would lead the research of GDH regulation from various organisms and they would contribute the elucidation of complicated regulatory mechanism of human GDH, which is involved in the important cell homeostasis.

Free Research Field

応用生物化学