2017 Fiscal Year Final Research Report
Production of novel prebiotics for intestinal bifidobacteria from food wastes
| Project/Area Number |
15K07448
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | ビフィズス菌 |
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| Outline of Final Research Achievements |
The production of prebiotics for intestinal bifidobacteria from two food wastes was studied.
(1) By using bifidobacterial glycosidases or cells, bifidogenic disaccharide galacto-N-biose was released from wasted mucin, which contains abundant O-glycans. A novel 6-sulfo-N-acetylglucosaminidase, an essential enzyme for degradation of terminally sulfated glycans, was identified. A bifidobacterial strain defective in incorporation of galacto-N-biose was also screened, which is suitable for production of the disaccharide.
(2) Mume-polyphenols prepared from umesu, a waste of umeboshi production, was found to enhance bifidobacterial growth. The major phenolic compound, prunose, was assimilated by B. catenulatum and B. pseudocatenulatum, which express prunose-specific ferulate esterase. Arabinan-like polysaccharides was degraded by two novel GH43 exo-α-arabinofuranosidases from B. longum. Effects of orally administrated mume-polyphenols on intestinal microbiota was evaluated using mice.
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| Free Research Field |
微生物学
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