Construction of the breeding system for all females production in kuruma prawn

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07566
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Fisheries Research and Education Agency
• Principal Investigator: FUSEYA Reiko 国立研究開発法人水産研究・教育機構, 水産工学研究所, 主任研究員 (40373469)
• Co-Investigator(Renkei-kenkyūsha): SAKAMOTO Takashi 国立大学法人 東京海洋大学, 海洋科学部, 教授 (40313390)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: クルマエビ / 育種システム / 人工交配 / 性連鎖マーカー / 閉鎖循環飼育

Outline of Final Research Achievements

The females of kuruma prawn grow faster and larger than the males. In this study, we constructed a single nucleotide polymorphism (SNP)-based genetic linkage map by the genotyping-by-sequencing (GBS) method, and identified the sex-determining locus. We established a simple artificial copulation method for breeding of kuruma prawn. Seed production was carried out using the artificial copulation of male and female with individual label. The SNP markers that were genotyped in at least 80% of the progenies exhibiting polymorphisms in the male or female parent of the mapping population were retained for subsequent genetic linkage map analysis. Three SNPs were linked to the sex determination locus. The sex-linked markers identified in this study should be useful in kuruma prawn breeding programs.

Free Research Field

水産増養殖学，集団生物学，集団遺伝学