2017 Fiscal Year Final Research Report
Studies on mapping of seminal proteins and regulation of calcium and protein tyrosine phosphorylation in relation to sperm flageller waveform and capacitation in the silkworm, Bombyx mori.
| Project/Area Number |
15K07793
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 精子成熟 / カイコガ / セリンプロテアーゼ / チロシンリン酸化 |
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| Outline of Final Research Achievements |
(1) The objective of this study was to evaluate the major basic protein profile of Bombyx seminal fluid and to determine if any of these proteins was associated with semen fertility. Six candidate proteins included Arg-Arg extended sequences in common. Moreover, they exhibited a step-wise regression with the progress of sperm maturation. These findings suggested that mainly the specific cleavage at Arg-Arg motif is caused by a trypsin-type protease, called initiatorin, which is thought to be involved in the sperm maturation. (2) We indicate that flagellar beating of silkworm sperm was controlled by Ca2+ levels, cAMP-dependent protein kinases (PKA) activities and the specific degradation of threonine-phosphorylated protein.
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| Free Research Field |
昆虫生殖・発生学
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