Air segmented - feedback based flow ratiometry for super high throughput titration

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07889
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: The University of Tokushima
• Principal Investigator: TANAKA Hideji 徳島大学, 大学院医歯薬学研究部(薬学系), 教授 (40207121)
• Co-Investigator(Kenkyū-buntansha): 竹内 政樹 徳島大学, 大学院医歯薬学研究部(薬学系), 准教授 (10457319)
• Research Collaborator: KAKIUCHI Naoya 徳島大学, 大学院薬科学教育部, 大学院生 ; KUBO Hiroya 徳島大学, 薬学部薬学科, 学生 ; OKA Sawako 徳島大学, 薬学部薬学科, 学生 ; HIRASAKA Tomoko 徳島大学, 薬学部薬学科, 学生 ; TOMIYAMA Erina 徳島大学, 薬学部薬学科, 学生 ; MIYAZAKI Aiko 徳島大学, 薬学部薬学科, 学生 ; Fujikawa Akihiro 四国理研有限会社
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: フローレイショメトリー / 流量比法 / 流れ分析 / フロー滴定 / 気節法 / ハイスループット滴定 / フィードバック制御 / 非水滴定

Outline of Final Research Achievements

Feedback-based flow ratiometry (FB-FR) is a sophisticated variation of flow analysis, first reported by Tanaka et al. in 2000. Flow ratio of sample and reagent solutions is continuously varied by variable and subsequent fixed triangular waves. Information on analyte is obtained by analyzing the relationship between sample/reagent flow ratio and detector output signal. Throughput rate can be increased by a feedback-based control.
In this study, air segmentation approach has been introduced to a flow titrimetry by FB-FR. Axial dispersion was limited in each liquid segment separated by air bubbles, resulting in the expansion of applicable range. The FB-FR system with unprecedented high throughput rate (max. 50 titrations/min, in non segmented mode) was also developed. FB-FR was further applied to a process control, where online analysis and control were carried out by coupling two kinds of feedback-based controls.

Free Research Field

分析化学