Functional and structural biological analysis of heterotrimeric G protein signal-dependent RhoGEF

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07927
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Gifu University
• Principal Investigator: UEDA Hiroshi 岐阜大学, 工学部, 教授 (50253779)
• Co-Investigator(Kenkyū-buntansha): 加藤 善一郎 岐阜大学, 大学院医学系研究科, 教授 (90303502)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Rho / RHoGEF / GPCR / G蛋白質 / チロシンキナーゼ / チロシンリン酸化 / 蛋白質間相互作用

Outline of Final Research Achievements

PLEKHG2 is a Rho family small G protein-specific guanine nucleotide exchange factor (RhoGEF) which is activated by the directly interaction with heterotrimeric G protein Gβγ subunits. However, there are no functional domains other than the Dbl homology (DH) domain and pleckstrin homology (PH) domain, which are active centers, in the structure of PLEKHG 2. In this study, functional regulation via interaction between molecules other than Gβγ subunits and PLEKHG2 was investigated. The interaction between PLEKHG2 and heterotrimeric G protein Gαs subunit, or ABL1, attenuate the activity of PLEKHG2. On the other hand, the interaction between PLEKHG2 and FHL1 activate the activity of PLEKHG2.

Free Research Field

生化学