Relation between the distribution of the stiffness of the LDL and age

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08607
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hokkaido University
• Principal Investigator: TAKEDA SEIJI 北海道大学, 保健科学研究院, 准教授 (80374726)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 低比重リポ蛋白質 / 硬さ / 酵素 / 加齢

Outline of Final Research Achievements

Oxidation of low-density lipoproteins (LDLs) is one of the risk factor for developing cardiovascular disease. Recently, we reported that the elastic modulus of oxidized LDLs decreased with increasing oxidation level of the LDL. However, the mechanisms of the decrease of the elastic modulus have not been well investigated. We thought that the structure change of the LDL might contributed to the decrease. This study measured the elastic modulus of LDLs before and after proteases, phospholipase A2. Although the enzyme treatment did not change the elastic modulus, treatment with PLA2 decreased the elastic modulus. Results suggest that structure change of the lipid structure of the LDL might contribute to the decreasing of the elastic modulus. We also investigated the relation between the age and the distribution of the LDL. Although detail more work will be required, we found that the elastic modulus tend to increase with increasing the age.

Free Research Field

生物物理化学