The potentiality of adipose tissue-derived stem cells in hematopoietic stem cell transplantation

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K09667
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Nippon Medical School
• Principal Investigator: HAYAKAWA JUN 日本医科大学, 医学部, 講師 (10386196)
• Co-Investigator(Kenkyū-buntansha): 植田 高弘 日本医科大学, 医学部, 准教授 (20322505)
• Research Collaborator: Uchimura Ryoichi
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: 脂肪幹細胞 / 間葉系幹細胞 / 血管炎 / 抗炎症効果

Outline of Final Research Achievements

Although Mesenchymal stem cells (MSCs) regulate in immune response and bone marrow recovery, adipose-tissue derived mesenchymal stem cells (ADSCs) are still not well defined. Similar possibility was expected by ADSCs as alternated tool of MSCs. The harvest of ADSCs was less invasive and more safe. We tried to evaluate the behavior of ADSCs in vivo mice model.
In vasculitis model, we demonstrated that ADSCs has anti-inflammatory and immune regulatory functions by suppressed cytokines such as IL-1b and TNF-α, IL10, IL-13, IL-17. Our pathological data also suggested ADSCs do not act directly on the site of inflammation, suppressing pro-inflammatory related cytokines causes reduction of vasculitis by acting indirectly through some factors.
Further studies required to clarify the role of ADSCs, ADSCs could potentially lead to unique tools in therapeutic strategies of vasculitis and bone marrow recovery.

Free Research Field

小児科学

Academic Significance and Societal Importance of the Research Achievements

安定した脂肪由来間葉系幹細胞(ADSC)を樹立し生態に投与することで生体内での働きを評価した。マウスから樹立した脂肪幹細胞をマウス血管炎モデルに投与したところ、ADSCが造血回復の際に血管炎の抑制に寄与することを示した。ADSCはIL-17やTNF-αといった炎症関連サイトカインを抑制することで冠動脈などの大動脈での炎症の改善に貢献し抗炎症効果をもたらすことを証明できた。しかし、本研究で目標とした骨髄不全症疾患などの造血幹細胞移植時における脂肪幹細胞の有用性の評価と造血回復への関与に関しての充分なdataは得ることができず今後の課題として残された。