2017 Fiscal Year Final Research Report
The regulation of focal mechanical stress on the localization of TRPV2 was investigated in the fibrosarcoma cells.
| Project/Area Number |
15K10019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 機械受容 / TRPV2チャネル / トランスロケーション / ビンキュリン / メカノセンサー |
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| Outline of Final Research Achievements |
The effect of focal mechanical stress on the localization of TRPV2 was investigated in HT1080 cells. When focal mechanical stress was applied, subplasma membrane Ca2+ concentration ([Ca2+]s) was increased beneath the pipette, which propagated throughout the cell. The increase in [Ca2+]s was blocked by knocking down TRPV2. The focal mechanical stress induced vinculin conformation change was blocked by knocking down TRPV2. Elevation of [Ca2+]s was not observed by the application of Gd3+ and mechanical stress induced accumulation of TRPV2 -GFP beneath the pipette was not observed.
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| Free Research Field |
細胞生物学
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