2017 Fiscal Year Final Research Report
Establishment of rapidly testing bacterial gene using PCR lateral flow assay in periprosthetic joint infection
| Project/Area Number |
15K10489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Research Collaborator |
SAKURAI KEIZOU 北里大学病院, 臨床検査部, 主任
NIHONYANAGI SHIN 北里大学病院, 臨床検査部・感染管理室
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 人工関節周囲感染 / PCRラテラルフロー法 / 迅速細菌遺伝子検査 / シーケンス解析 / 16S-rRNA / MRSA |
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| Outline of Final Research Achievements |
The gold standard for diagnosing periprosthetic joint infections (PJI) is bacterial culture. Unfortunately, it usually takes a few days for the results of these cultures to be obtained. In this study, the objective was to determine whether PCR-lateralflow (PCR-LF) tests could be used to effectively diagnose PJI. We used PCR-LF to diagnose common causes of PJI and compared the results to those from conventional bacterial cultures of the same samples. Two genetic markers specific to MRSA were being detected during this analysis. PCR results were interpreted as follows MRSA, MSSA, MR-CNS or negative. Our data indicate that PCR-LF was superior to bacterial culture tests in terms of sensitivity, particularly for PJI samples. In conclusion, our results showed that PCR-LF is a useful tool for identifying pathogenic bacteria in PJI.
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| Free Research Field |
整形外科
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