Effects of GLP-1 and/or volatile anesthetics against ischemia-reperfusion injury under hyperglycemia

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K10513
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: The University of Tokushima
• Principal Investigator: SAKAI Yoko 徳島大学, 病院, 特任准教授 (90711862)
• Co-Investigator(Kenkyū-buntansha): 田中 克哉 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (30263841) ; 堤 保夫 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (90523499)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 高血糖 / インクレチン / 虚血再灌流障害 / イソフルラン / 心筋保護作用

Outline of Final Research Achievements

Glucagon-like peptide-1 receptor (GLP-1R) agonist and volatile anesthetic isoflurane protects the heart from ischemia/reperfusion injury. We tested the hypothesis that hyperglycemia interferes with GLP-1 agonist and/or isoflurane-induced cardiac protection affect using in vitro and in vivo studies. Myocytes were treated with GLP-1 and/or isoflurane. This was then followed by simulated ischemia/reperfusion (SI/R). In addition, cardiac protection in vivo was assessed by measuring infarct size and cardiac troponin levels. GLP-1 and isflurane protected cardiac myocytes from SI/R but this protection was bolished by hyperglycemia. Furthermore, GLP-1 and/or isoflurane induced a reduction in infarct size and cardiac troponin relative to control. However, induced cardiac protection was abolished in hyperglycemia. These results indicate tha blood glucose are important determinants of infarct size during GLP-1 and isoflurane-induced cardiac protection.

Free Research Field

麻酔科学