2017 Fiscal Year Final Research Report
Anti-oxidative stress pathway in the ovary to conquer inevitable aging
Project/Area Number |
15K10704
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Obstetrics and gynecology
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Research Institution | The University of Tokyo |
Principal Investigator |
Hiraike Osamu 東京大学, 医学部附属病院, 准教授 (20529060)
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Research Collaborator |
HIRANO Mana 東京大学, 医学部附属病院, 助教 (80771253)
AKINO Nana
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | 卵巣 / 加齢性変化 / 酸化ストレス / 抗酸化機能 |
Outline of Final Research Achievements |
Nuclear factor-E2-related factor 2 (Nrf2) and its regulator Kelch-like ECH-associated protein 1 (Keap1) are one of the most important defense mechanisms against oxidative stress (OS). Dimethylfumarates (DMF) is an activator of Nrf2, and we investigated the possibility of DMF as a therapeutic drug for OS in human granulosa cells (GCs). We aimed to determine whether DMF alleviates OS in GCs. The mRNA and protein levels of Nrf2 and antioxidants were upregulated by H2O2 in human GCs, and DMF treatment showed similar results through activation of Nrf2. Knockdown of Nrf2 decreased the mRNA and protein levels of antioxidants in human GCs. DMF treatment of GCs activated Nrf2 and reduced intracellular ROS generation. Our results show that activation of Nrf2 by DMF might lead to alleviated OS in human GCs, and this will lead to better outcomes in infertility treatment.
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Free Research Field |
産婦人科学
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