2017 Fiscal Year Final Research Report
Molecular biological analysis on periodontal disease and obesity centered on fat metabolism analysis of bacterial invaded macrophages
| Project/Area Number |
15K11083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | インフラマソーム / マクロファージ / ピロトーシス / オメガ3系脂肪酸 / サイトカイン |
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| Outline of Final Research Achievements |
We clarified the role of ω-3 fatty acids in periodontopathic bacteria-invaded macrophages. Human macrophage cell line THP-1 was invaded with Periodontopathic bacterium. To make knock down cells, siRNA was induced to macrophages by electroporation. Periodontopathic bacteria invasion induced the expression of IL-1β and gasdermin D in THP-1 cells, concurrently with cell death. DHA downregulated cell death and the secretion of IL-1β from periodontopathic bacteria-invaded THP-1 cells. DHA also downregulated the expression of inflammasome-related protein induced by periodontopathic bacteria invasion in THP-1 cells. Depletion of caspase-4 downregulated the expression of IL-1β and gasdermin D in periodontopathic bacteria-invaded THP-1 cells. These results indicate that ω-3 fatty acid, particularly DHA, downregulated the inflammasome activity in THP-1 cells induced by periodontopathic bacteria through the downregulation of ASC and caspase-4 expression.
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| Free Research Field |
感染症学・免疫学
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