Development of novel dental pulp preservation and calcification therapies by analyzing epigenetics and post-transcriptional regulation mechanism

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: YUMOTO Hiromichi 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (60284303)
• Co-Investigator(Kenkyū-buntansha): 松尾 敬志 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (30173800) ; 中西 正 徳島大学, 大学院医歯薬学研究部(歯学系), 准教授 (00217770) ; 平尾 功治 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (00581399)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 象牙芽細胞 / 自然免疫 / 遺伝子発現 / シグナル伝達 / ポリフェノール / ケモカイン / VEGF

Outline of Final Research Achievements

Odontoblast-like cell line (KN-3) enhanced chemokines production through the innate immune receptor NOD1 and p38-AP-1 signaling pathway. Caffeic Acid Phenethyl Ester, which is one of polyphenols, reduced their production but induced VEGF production even under calcification induction conditions. Regarding the innate immune response, tolerance was observed in both odontoblasts and dental pulp fibroblasts. microRNAs (miRNAs) involve in the regulation of gene post-transcription and miRNAs whose expression was changed 2-fold in KN-3 cells were identified. These findings suggest that the applications of CAPE and post-transcriptional regulation, such as miRNA, may be useful for dental pulp preservation and calcification / regeneration therapies.

Free Research Field

医歯薬学