Molecular mechanisms of DEC-Twist transcription factors in oral squamous cell carcinoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11274
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: BHAWAL Ujjal 日本大学, 松戸歯学部, 助教 (50433339)
• Co-Investigator(Kenkyū-buntansha): 谷本 圭司 広島大学, 原爆放射線医科学研究所, 助教 (90335688)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 口腔扁平上皮癌 / 転写因子 / DEC1 / Twist1

Outline of Final Research Achievements

We have analyzed Twist1 transcriptional regulatory mechanism by forced expression of DEC1 using Luciferase reporter assay. Twist1 promoter including E-box was used. Twist1 transcriptional activity was gradually suppressed with DEC1 expression plasmid in a concentration-dependent manner. Mutation in the E-box did not affect Twist1 transcriptional activity. Protein expression of Twist1 and DEC1 was evaluated using Western blotting. Furthermore, we have investigated the binding of DEC1 with the E-box of Twist1 using Chromatin immunoprecipitation (ChIP assay). Compared with the control, the precipitate amount of Twist1 was increased due to forced expression of DEC1. In addition, the amount of precipitating Twist1 was suppressed by introducing DEC1 siRNA into cancer cells using the ChIP assay.

Free Research Field

医歯薬学