Development of new molecular target chemotherapy using in vitro oral carcinogenesis model

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11284
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hyogo Medical University
• Principal Investigator: Zushi Yusuke 兵庫医科大学, 医学部, 研究生(研究員) (80581206)
• Co-Investigator(Kenkyū-buntansha): 岸本 裕充 兵庫医科大学, 医学部, 教授 (30291818) ; 野口 一馬 兵庫医科大学, 医学部, 准教授 (50309473)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: in vitro 発癌 / 病態モデル / がん化学療法 / 分子標的薬 / 薬剤耐性

Outline of Final Research Achievements

We try to develop new molecular target chemotherapy against metastasis related gene and/or cancer stem cell related gene using in vitro oral carcinogenesis model with immortalized human tongue keratinocyte transfected cell lines.
At first, we established anti-cancer durg resistant cell line of CDDP or Cetuximab , that was used for oral cancer chemotherapy. As results, human papilloma virus (HPV) related oral cancer cell line, and immortalized human tongue keratinocyte transfected cell lines did not develop to anti-cancer resistance. Non-HPV related oral cancer cell lines, for example, SCCKN, and HSC-3, got resistant for CDDP and Cetuximab.
We analyzed gene mutation between parental and resistant cell lines by next generation sequencer. We indicate there is no relation of gene mutation and copy number of of ABC transporter proteins or EGFR. Most imortant point toget resistance for anti-cancer drugs is gene mutation of AKT or PI3K.

Free Research Field

口腔癌