Development of a light enhancement plate for Raman spectroscopy

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K12519
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAKAMATSU TETSURO 京都府立医科大学, 医学(系)研究科(研究院), 教授 (40154900)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ラマン散乱分光 / 分子イメージング / バイオイメージング

Outline of Final Research Achievements

Raman scattering spectroscopy is a promising technique to evaluate cells and tissues without any treatment such as fixation and staining based on molecular vibrations of inherent molecules of samples. However, the conventional Raman spectroscopy is time-consuming and has low molecular sensitivity due to the low efficiency of Raman scattering, disabling sufficient cell and tissue diagnosis. To overcome these issues, we developed a novel Raman spectroscopy with a light enhancement plate involving a plasmonic enhancement effect. We realized enhanced Raman imaging of nerves, muscles, connective tissues, adipose tissues with high enhancement factor. Our approach would be a useful and powerful tool for label-free diagnosis of cells and tissue for biological and medical applications.

Free Research Field

生体医工学・生体材料学