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2015 Fiscal Year Final Research Report

Molecular breeding of anmonia-producing bacteria

Research Project

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Project/Area Number 15K14233
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionKyoto University

Principal Investigator

Ueda Mitsuyoshi  京都大学, (連合)農学研究科(研究院), 教授 (90183201)

Project Period (FY) 2015-04-01 – 2016-03-31
Keywordsプロテオーム解析 / アンモニア生産育種 / 液体水素エネルギー
Outline of Final Research Achievements

Rhizobia are soil bacteria that fix nitrogen under the symbiotic condition with host legumes. In the root nodule, rhizobia differentiate into a bacteroid, a symbiosis-specific form, and then they fix nitrogen and provide ammonia as a nitrogen source to host plant. Many proteins other than nitrogenase complex are involved in nitrogen fixation. In this study, we performed quantitative proteome analysis during nodule development to elucidate the mechanism of bacteroid differentiation. Quantitative proteome profiling showed that the proteins encoded on the symbiosis island were up-regulated under the symbiotic condition. Moreover, time course analysis indicated that nitrogenase complex was constructed after the construction of its redox centers, such as FeMo cofactor and 4Fe:4S cluster. Our quantitative proteome analysis can be helpful for clarification of mechanism of nitrogen fixation。

Free Research Field

応用生物科学

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Published: 2017-05-10  

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