Development of applicable methods for advanced gene targeting on mouse Y chromosome

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14369
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: The University of Tokushima
• Principal Investigator: MATSUMOTO Takahiro 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (70447374)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: Y染色体

Outline of Final Research Achievements

The Y chromosome is male-specific chromosome and considered to be critical for sexual differentiation and male physiology and behavior. However, its physiological impact has been elusive, owing to the lack of a mouse genetic model. The targeted disruption of the Y-linked genes in mouse ES cells has not been successful, because conventional homologous recombination is hampered by the unique structural features of the Y chromosome. We have overcome this problem and generated floxed mouse line, by using a site-directed insertion vector and CRISPR/Cas9 system. Moreover, we developed multi-copy Y gene knockdown transgenic mice with co-cistronic expression of three miRNA hairpins.

Free Research Field

分子生物学