Single molecule analysis in the generation of oscillatory circuit for cAMP signaling

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14491
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: The University of Tokushima
• Principal Investigator: HORIKAWA Kazuki 徳島大学, 大学院医歯薬学研究部(医学系), 教授 (70420247)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: イメージング / 生物リズム / 自己組織化 / cAMP

Outline of Final Research Achievements

To ask the mechanisms in the construction of oscillatory signaling circuit, we performed single molecule quantification for essential components in the cAMP signaling system. We developed essential technologies including the efficient generation of GFP-tagged cell lines(BMC Biotech, 2016) and ultrasensitive cAMP indicators (ACS Chem Biol, 2016). By utilizing these tools, we performed a simultaneous imaging of cellular function (cAMP) and molecular quantification and identified that self-organized construction of signaling circuit is regulated by few proteins characterized with significantly low level expression.

Free Research Field

生物物理