Regulation of intracellular Ca2+ concentration by ORAI-2 localized on mast cell secretory granules.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K14495
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Kanazawa University (2017); Nagoya City University (2015-2016)
• Principal Investigator: Suzuki Ryo 金沢大学, 薬学系, 教授 (00344458)
• Co-Investigator(Renkei-kenkyūsha): HIRASHIMA NAOHIDE 名古屋市立大学, 大学院薬学研究科, 教授 (10192296)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 開口放出 / カルシウムチャネル / アレルギー / マスト細胞 / 分泌小胞

Outline of Final Research Achievements

To investigate the molecular mechanism of ORAI-2 localized on mast cell secretory granules, we tried to observe localization of ORAI isoforms (ORAI-1 and -2) in tissue mast cells. We found that ORAI-2 were localized on secretory granules although ORAI-1 were localized in the plasma membrane in tissue mast cells. Next, we examined the interaction of ORAI-2 proteins with STIM and tubulin. Co-immunoprecipitation experiment revealed that interaction of ORAI-2 with STIM and tubulin were increased after IgE/antigen stimulation. We also found that ORAI-2 localized on mast cell secretory granules played an important role in exocytosis by fluorescent protein-based biosensor analysis.

Free Research Field

アレルギー