Analysis of alternative splicing regulated by CHERP

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K14919
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyoto University
• Principal Investigator: Masuda Seiji 京都大学, 生命科学研究科, 准教授 (20260614)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 選択的mRNAスプライシング / CHERP / カルシウム / 遺伝子発現

Outline of Final Research Achievements

Alternative mRNA splicing is a fundamental mechanism to produce plenty of protein more than those encoded in its genome. The understanding of alternative splicing will make us to develop the therapy strategy for diseases based on the inadequate miss-splicing.
CHERP is first identified as Ca2+ signaling related protein in the endoplasmic reticulum. Then, CHERP is also localized in the nucleus, suggesting CHERP has another function. Here, I examined the CHERP regulating genes and alternative splicing. To analyze the genome wide expression change of each mRNA and exon, the exon array analysis was performed. To know the function of CHERP, then, GO term analysis was also carried out. The depletion of CHERP caused the decreased expression of cell cycle, mitosis, cytokinesis, meiosis, DNA repair and DNA replication GO term and increased the induction of apoptosis GO term, suggesting that CHERP has a role for cell proliferation and survival by regulating mRNA splicing.

Free Research Field

応用分子細胞生物学