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2017 Fiscal Year Final Research Report

Development of in vitro culture system for expansion of Leydig stem cells

Research Project

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Project/Area Number 15K15013
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionKyoto University

Principal Investigator

Shinohara Mito  京都大学, 医学研究科, 助教 (10372591)

Project Period (FY) 2015-04-01 – 2018-03-31
Keywords幹細胞 / 生殖 / 精子
Outline of Final Research Achievements

Recent studies suggest a possibility that not only germ cell, but Leydig cells of testes is supported by stem cell system. This study aimed at the establishment of in vitro culture system to maintain and expand Leydig stem cell for a long-term period. To enrich Leydig stem cell population, we isolated side population (SP) fraction from testes cells, based on the ability of stem cells to effectively exclude the dye Hoechst 33342. After SP fraction was cultured with serum-free medium for 2 weeks, a part of the cultured cells expressed PDGFRA, a marker of Leydig cells. Cultured cells derived from Green mice, which ubiquitously express EGFP, were dissociated by enzyme digestion and injected into the interstitial space of wild-type mouse testes. At 3 months after injection, host testes had EGFP expressing cells, which was detected by UV light excitation, however, immunofluorescence study showed that Leydig cell marker PDGFRA was not detected in EGFP expressing cells.

Free Research Field

生殖医学

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Published: 2019-03-29  

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