2016 Fiscal Year Final Research Report
Critical mechanisms for the determination of hepatocytic and bile ductular differentiation
Project/Area Number |
15K15107
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
|
Research Institution | Asahikawa Medical College |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | 肝細胞 / 胆管上皮細胞 / 分化転換 / HNF-4α / 転写因子 / 転写調節 |
Outline of Final Research Achievements |
Transdifferentiation of hepatocytes into bile duct cells is associated with the switching of the expression of P1-dependent isoforms to P2-dependent isoforms of HNF-4α. Although the P1 promoter has been suggested to be regulated by COUP-TF and CTIP, the expression of these transcription factors was increased only slightly and at a later time point of transdifferentiation and theirby their expression was not likely to be the mechanism of the switching of the promoter usage. Our experiments using a Notch inhibitor (DAPT) and a transposon-mediated introduction of a Notch intracellular domain suggested that the Notch signaling was involved in the activation of the P2 promoter as well as hepatocytic transdifferentiation.
|
Free Research Field |
実験病理学
|