Three-dimensional culture of reprogrammed brown adipocytes

2015 Fiscal Year Final Research Report

• Project/Area Number: 15K15111
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Mazda Osam 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00271164)
• Co-Investigator(Renkei-kenkyūsha): Kawahito Yutaka 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (50336731) ; Gojo Satoshi 京都府立医科大学, 医学(系)研究科(研究院), 教授 (90316745) ; Kishida Tsunao 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205)
• Project Period (FY): 2015-04-01 – 2016-03-31
• Keywords: 糖尿病

Outline of Final Research Achievements

We established a procedure to directly convert fibroblasts into brown adipocytes. We also found that transplantation of the brown adipocytes remarkably suppressed diabetes in mice. Three-dimensional culture of the brown adipocytes may further improve the viability and metabolic activity of the cells in vivo after transplantation.
Then we aimed at forming 3D brown adipose tissue using the reprogrammed brown adipocytes. After various trials, we observed some characteristic structure by co-culturing different lineages of the cells. The present findings may implicate a novel tissue engineering procedure that may be useful for regenerative therapy for diabetes mellitus.

Free Research Field

再生医学