To understand molecular bases of how intraluminal pressure elevation leads to the development of degenerative lesions

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K15113
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Kindai University
• Principal Investigator: ITO Akihiko 近畿大学, 医学部, 教授 (80273647)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 内圧上昇 / 変性 / 緑内障 / 接着分子 / メカノバイオロジー / メカノパソロジー / two-chamber培養装置

Outline of Final Research Achievements

Elevation of intraluminal, such as intraocular and intra-renal pelvic, pressure causes neuronal and mucosal degeneration. The threshold is fairly low and constant, approximately 30 cmH2O. A new two-chamber culture system was devised wherein cells were cultured on a semipermeable membrane and subjected to increased medium height (water pressure up to 60 cmH2O). Primary cultured mouse neurons were found to fall into axonal degeneration when loaded with 30 cmH2O or more, in association with increased ectodomain shedding of CADM1, an IgCAM member expressed on neuritic cell membrane. The cell-side remnant produced by shedding appeared to be involved in axonal degeneration. Columnar epithelial cells were flattened and growth suppressed when loaded with 30 cmH2O or more. This work contributes to our understanding the pathophysiology of the development of degenerative lesions caused by low static pressure burden.

Free Research Field

病変解析の実験病理学、接着分子の病態学