2017 Fiscal Year Final Research Report
Generation of antigene-fixed nanifibers and its application to autoantibody detection
Project/Area Number |
15K15190
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory medicine
|
Research Institution | Kanazawa University |
Principal Investigator |
Yokoyama Shigeru 金沢大学, 子どものこころの発達研究センター, 教授 (00210633)
|
Co-Investigator(Renkei-kenkyūsha) |
YOSHIKAWA Hiroaki 金沢大学, 保健管理センター, 教授 (10272981)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Keywords | 膜タンパク / 自己抗体 / ナノファイバー |
Outline of Final Research Achievements |
In autoimmune neuropsychiatric diseases, neurotransmitter receptors and ionic channels are known to be targeted by autoantibodies that destruct neural tissues. In an attempt to establish a detection systems for autoantibodies, we produced these proteins in Escherichia coli. Of several proteins tried, two subunits for the glutamate receptor, GluN1-1 and GRIA2, and a neuronal nicotinic acetylcholine receptor, nAchR-alpha7 were successfully produced in large amount. We are now trying to find out media to dissolve these recombinant proteins and optimal conditions to integrate them into nanofibers.
|
Free Research Field |
神経科学
|