Response of skeletal muscle in mice and hamster to ketogenic diet feeding

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K16499
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: The University of Tokushima (2017); National Institute of Advanced Industrial Science and Technology (2015-2016)
• Principal Investigator: NAKAO Reiko 徳島大学, 大学院医歯薬学研究部(医学系), 講師 (20582696)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ケトン体食 / 熱産生

Outline of Final Research Achievements

We found that the mRNA expression of Slc25a25, a Ca2+-sensitive ATP carrier in the inner mitochondrial membrane, fluctuates in a circadian manner in mouse skeletal muscle and it was damped in Clock mutant and Bmal1-deficient mice. Furthermore, a ketogenic diet (KD) that induces time-of-day-dependent hypothermia, induced Slc25a25 mRNA expression in skeletal muscle. Sciatic denervation abolished circadian and KD-induced Slc25a25 expression. We measured body temperature (Tb) in sciatic denervated mice fed with KD to determine the functional role of KD-induced Slc25a25 expression. Sciatic denervation abolished Slc25a25 expression and augmented KD-induced hypothermia compared with sham-operated mice. These findings suggest that KD feeding induces expression of the muscle circadian gene Slc25a25 via neural pathways, and that SLC25A25 might be involved in muscle thermogenesis under KD-induced hypothermia in mice.

Free Research Field

栄養学