2016 Fiscal Year Final Research Report
Regeneration of cochlear hair cell by transplantation of Multilineage-differentiating Stress Enduring (Muse) cell
| Project/Area Number |
15K20179
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Research Collaborator |
KAKEHATA Seiji
ITOU Tsukasa
KOIZUMI Yutaka
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Muse細胞 / 内耳障害 / 内耳保護 |
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| Outline of Final Research Achievements |
First I made a inner ear degeneration model by band noise exposure. Noise frequency was from 4 to 8 kHz and its sound presser was 130 dBSPL. This condition can make a permanent threshold shift of hearing. I transplanted 10,000 Muse cells into scala tympani of this model using micro catheter 1 day after noise exposure. the hearing threshold recovered significantly on Muse transplanted ear at the area of 4 and 8 kHz from 6 weeks after noise exposure to the end point.I counted the rest of outer hair cell at 12 weeks after noise exposure, this revealed that more outer hair cells were alive in Muse transplanted group than control at 8 kHz area with a statistically significant difference. Now we are investigating whether Muse cells can migrate and engraft to the damaged organ of Corti and differentiate into hair cells or hair cell progenitor cells.
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| Free Research Field |
耳科学
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