2016 Fiscal Year Final Research Report
Investigation of uPA/uPAR induced EMT in ARPE-19
| Project/Area Number |
15K20294
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kindai University |
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Principal Investigator |
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| Research Collaborator |
SUGIOKA Koji 近畿大学, 医学部, 講師 (50399119)
YOSHIDA Koji 近畿大学, 生物理工学部, 教授 (60230736)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | uPA / uPAR / ARPE-19 / EMT |
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| Outline of Final Research Achievements |
In EMT (epithelial-messenchymal transition) study, retinal pigment epithelial cells have been the main research subject and findings of EMT on retinal pigment epithelial cells have been accumulated. Contrarily, there are few reports, so far, on EMT induced by uPAR through the fibrinolytic system. Therefore, in this study, uPAR was stably introduced into human retinal pigment epithelial cells (ARPE-19), and EMT induced by uPAR was investigated. In consequence, EMT-like changes could not be recognized in ARPE-10 by stably introduced uPAR alone, and collagen gel contraction could not be recognized, either. EMT could not be induced in the retina with a single stimulation of increased expression of uPAR. Involvement of growth factors like TGF-β and cytokines like interleukin should also be considered in the future study.
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| Free Research Field |
眼科領域
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