Dynamics of synapse and exocytosis studied with two-photon excitation

2008 Fiscal Year Final Research Report

• Project/Area Number: 16002012
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo (2005-2008); National Institute for Physiological Sciences (2004)
• Principal Investigator: KASSAI Haruo The University of Tokyo, 大学院・医学系研究科, 教授 (60224375)
• Project Period (FY): 2004 – 2008
• Keywords: 生物・生体工学 / シグナル伝達 / 生理学 / 糖尿病 / 脳・神経

Research Abstract

Using two-photon microscope and uncaging of caged-glutamate compounds, we have revealed that rapid motilities of dendritic spines underlie plasticity of excitatory synaptic transmission in the cerebral cortex. We have visualized how actin reorganization underlies the motilities, and how spine sizes can be maintained over days even though the synapses are living small structures, and cannot avoid the natural fluctuations. We have developed a new caged-GABA compound, which can be used simultaneously with caged-glutamate compound. A new principle for visualization of exocytosis with two-photon microscope has been established, and applied to analyze conformational changes of responsible molecules.

Research Products

• [Journal Article] SNARE conformational changes that prepare vesicles for exocytosis. (2010)
  • Author(s): Takahashi, N., Hatakeyama, H., Okado, H., Noguchi, J. Ohno, M., Kasai, H.
  • Journal Title: Cell Metabolism (In press)
  • Peer Reviewed
• [Journal Article] 1-Acyl-5-methoxy-8-nitro-1, 2-dihydroquinoline: A biologically useful photolabile precursor of carboxylic acids. (2010)
  • Author(s): Obi, N., Momotake, A., Kanemoto, Y., Matsuzaki, M, Kasai, H., Arai, T.
  • Journal Title: Tetrahedron letters 51 Pages: 1642-1657
  • Peer Reviewed
• [Journal Article] Two-color, two-photon uncaging of glutamate and GABA. (2010)
  • Author(s): Kantevari, S. *, Matsuzaki, M. *, Kanemoto, Y., Kasai, H. CA, Ellis-DaviesG.C.R. CA
  • Journal Title: Nature Methods 7 Pages: 123-125
  • Peer Reviewed
• [Journal Article] Two photon uncaging of γ-aminobutyric acid probes on neurons in intact brain tissue. (2010)
  • Author(s): Matsuzaki, M. Hayama, T., Kasai, H., Ellis-Davies, G.C. R
  • Journal Title: Nature Chemical Biology 6 Pages: 255-257
  • Peer Reviewed
• [Journal Article] Structural dynamics of dendritic spines in memory and cognition. (2010)
  • Author(s): Kasai, H., Fukuda, M, Watanabe, S., Hayashi-Takagi, A., Noguchi, J.
  • Journal Title: Trends Neurosci 33 Pages: 121-129
  • Peer Reviewed
• [Journal Article] Exocytosis in islet beta-cells. (2010)
  • Author(s): Kasai, H., Hatakeyama, H., Ohno, M., Takahashi, N.
  • Journal Title: Advances in Experimental Medicine and Biology 654 Pages: 03-336
  • Peer Reviewed
• [Journal Article] Generation, elimination and weight fluctuations of synapses in the cerebral cortex. (2009)
  • Author(s): Morita, S., Yasumatsu, N. Noguchi, J., Kasai, H.
  • Journal Title: Communicative and Integrative Biology 2 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Transcranial optogenetic stimulation for functional mapping of the motor cortex. (2009)
  • Author(s): Hira, R., Honkura, N., Noguchi, J., Maruyama, Y., Augustine, G.J., Kasai, H., Matsuzaki, M.
  • Journal Title: J. Neurosci. Methods 179 Pages: 258-263
  • Peer Reviewed
• [Journal Article] Principles of long-term dynamics of dendritic spines. (2008)
  • Author(s): Yasumatsu, N., Matsuzaki, M., Miyazaki, T., Noguchi, J., Kasai, H.
  • Journal Title: J. Neurosci. 28 Pages: 13592-13608
  • Peer Reviewed
• [Journal Article] Protein synthesis and neurotrophin-dependent structural plasticity of single dendritic spines. (2008)
  • Author(s): Tanaka, J., Horiike, Y., Matsuzaki, M., Miyazaki, T., Ellis-Davies, GCR, Kasai, H.
  • Journal Title: Science 319 Pages: 1683-1687
  • Peer Reviewed
• [Journal Article] Three-dimensional 5 mapping of unitary synaptic connections by two-photon macro photolysis of caged glutamate. (2008)
  • Author(s): Matsuzaki., M., Ellis-Davies, G.C.R., Kasai, H.
  • Journal Title: J. Neurophysiol. 99 Pages: 1535-1544
  • Peer Reviewed
• [Journal Article] The subspine organization of actin fibers regulates the structure and plasticity of dendritic spines. (2008)
  • Author(s): Honkura, N., Matsuzaki, M., Noguchi, J., Ellis-Davies, G.C.R., Kasai, H.
  • Journal Title: Neuron 57 Pages: 719-729
  • Peer Reviewed
• [Journal Article] Exocytic process analyzed with two-photon excitation imaging in endocrine pancreas. (2007)
  • Author(s): Takahashi, N., Kasai, H.
  • Journal Title: Endocrine J. 54 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Two cAMP-dependent pathways differentially regulate large-dense core and small vesicles in mouse beta cells. (2007)
  • Author(s): Hatakeyama, H., Takahashi, N., Kishimoto, T., Nemoto, T., Kasai, H.
  • Journal Title: J. Physiol. 582 Pages: 1087-1098
  • Peer Reviewed
• [Journal Article] CDNI-Glu: an improved caged glutamate for expeditious ultraviolet and 2-photon photolysis in brain slices. (2007)
  • Author(s): Ellis-Davies, G.C.R., Matsuzaki, M., Paukert, M., Kasai, H., Bergles, D.E.
  • Journal Title: J. Neurosci. 27 Pages: 6601-6604
  • Peer Reviewed
• [Journal Article] Activation and high-speed mapping of brain circuitry using photostimulation in Channelrhodopsin-2 transgenic mice. (2007)
  • Author(s): Wang, H. *, Peca, J. *, Matsusaki, M. *, Matsuzaki, K., Noguchi, J., Qiu, L., Wang, D., Zhang, F., Boyden, E., Deisseroth, K., Kasai, H., Hall, W.C., Feng, G., Augustine, G.J.
  • Journal Title: PNAS 104 Pages: 8143-8148
  • Peer Reviewed
• [Journal Article] Two-photon excitation imaging of exocytosis and endocytosis and determination of their spatial organization. (2006)
  • Author(s): Kasai, H., Kishimoto, T. Nemoto, T. Hatakeyama, H, Liu, T-T., Takahashi, N.
  • Journal Title: Adv. Drug Delivery Rev. 58 Pages: 850-877
  • Peer Reviewed
• [Journal Article] Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla. (2006)
  • Author(s): Kishimoto, T., Kimura, R., Liu, T. -T., Nemoto, T., Takahashi, N., Kasai, H.
  • Journal Title: EMBO J. 25 Pages: 673-682
  • Peer Reviewed
• [Journal Article] Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets. (2006)
  • Author(s): Hatakeyama, H., Kishimoto, T., Nemoto, T., Kasai, H., Takahashi, N.
  • Journal Title: J. Physiol. 570 Pages: 271-282
  • Peer Reviewed
• [Journal Article] Exocytosis and endocytosis of small vesicle in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis. (2005)
  • Author(s): Liu, T. -T., Kishimoto, T., Hatakeyama, H., Nemoto, T., Takahashi, N., Kasai, H.
  • Journal Title: J. Physiol. 568 Pages: 917-929
  • Peer Reviewed
• [Journal Article] Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis. (2005)
  • Author(s): Kishimoto, T., Liu, T. -T., Hatakeyama, H., Nemoto, T., Takahashi, N., Kasai, H.
  • Journal Title: J. Physiol. 568 Pages: 905-915
  • Peer Reviewed
• [Journal Article] A new quantitative (two-photon extracellular polar-tracer imaging-based quantification (TEPIQ)) analysis for diameters of exocytic vesicles and its application to pancreatic islets. (2005)
  • Author(s): Kasai, H., Hatakeyama, H., Kishimoto, T., Liu, T. -T., Nemoto, T., Takahashi, N.
  • Journal Title: J. Physiol. 568 Pages: 891-903
  • Peer Reviewed
• [Journal Article] Spine-neck geometry determines NMDA receptor-dependent Ca^2+ signaling in dendrites. (2005)
  • Author(s): Noguchi, J., Matsuzaki, M., Ellis-Davies, G.C.R., Kasai, H.
  • Journal Title: Neuron 46 Pages: 609-622
  • Peer Reviewed
• [Journal Article] Rapid Ca2+-dependent increase in oxygen consumption by mitochondria in single mammalian central neurons. (2005)
  • Author(s): Hayakawa, Y., Nemoto, T., Iino, M., Kasai, H.
  • Journal Title: Cell Calcium 37 Pages: 359-370
  • Peer Reviewed
• [Journal Article] Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands. (2005)
  • Author(s): Oshima, A., Kojima, T., Dejima, K., Hisa, I., Kasai, H., Nemoto, T.
  • Journal Title: Cell Calcium 37 Pages: 349-357
  • Peer Reviewed
• [Journal Article] Number and Density of AMPA Receptors in Single Synapses in Immature Cerebellum. (2005)
  • Author(s): Tanaka, J., Matsuzaki, M., Tarusawa, E., Momiyama, A., Molnar, E., Kasai, H., Shigemoto, R.
  • Journal Title: J. Neurosci. 25 Pages: 799-807
  • Peer Reviewed
• [Journal Article] Two-photon uncaging microscopy (2004)
  • Author(s): Kasai, H., Matsuzaki, M., Ellis-Davies, G.C.R.
  • Journal Title: In "Imaging in Neuroscience and Development, " CSH Lab (Press)
  • Peer Reviewed
• [Journal Article] Stabilization of exocytosis by dynamic F-actin coating of zymogen granules in pancreatic acini. (2004)
  • Author(s): Nemoto, T., Kojima, T., Oshima, A., Bito, H., Kasai, H.
  • Journal Title: J. Biol. Chem 279 Pages: 37544-37550
  • Peer Reviewed
• [Journal Article] Structural basis of long-term potentiation in single dendritic spines. (2004)
  • Author(s): Matsuzaki, M., Honkura, N., Ellis-Davies, G.C.R., Kasai, H.
  • Journal Title: Nature 429 Pages: 761-766
  • Peer Reviewed
• [Presentation] Two-photon imaging of brain cell dynamics (2009)
  • Author(s): H. Kasai
  • Organizer: Two-photon uncaging in vitro and in vivo. EMBO Practival course in 6
  • Place of Presentation: Zurich, Swiss
  • Year and Date: 2009-09-09
• [Presentation] Protein synthesis and neurotrophin-dependent structural plasticity of dendritic spines. (2009)
  • Author(s): H. Kasai
  • Organizer: Neurotrophic Factor Gordon Research Conference
  • Place of Presentation: Rhode Island, USA
  • Year and Date: 2009-06-23
• [Presentation] Patch-clamp, imaging and photostimulation. (2009)
  • Author(s): H. Kasai
  • Organizer: Third Neurizons Conference
  • Place of Presentation: Goettingen, Germany
  • Year and Date: 2009-05-15
• [Presentation] Two-photon imaging, uncaging and photoactivation of dendritic spines. (2008)
  • Author(s): H. Kasai
  • Organizer: Jacques Monod Conference "Investigating brain function using light
  • Place of Presentation: Roscoff, France
  • Year and Date: 2008-06-22
• [Presentation] Imaging of exocytosis and endocytosis with two-photon microscope. (2008)
  • Author(s): H. Kasai
  • Organizer: International Meeting on Exocytosis
  • Place of Presentation: Ljublijana, Slovenia
  • Year and Date: 2008-05-23
• [Presentation] Principles of long-term dynamics of dendritic spines. (2008)
  • Author(s): H. Kasai
  • Organizer: Neuroscience Workshop in NUS
  • Place of Presentation: Singapore
  • Year and Date: 2008-04-21
• [Presentation] Actin-based plasticity of dendritic spines. (2008)
  • Author(s): H. Kasai
  • Organizer: US-Japan Workshop
  • Place of Presentation: Asilomar, USA
  • Year and Date: 2008-02-26
• [Presentation] Structure dependence of plasticity in dendritic spines. (2006)
  • Author(s): H. Kasai
  • Organizer: Gordon Research Conference on Synaptic transmission.
  • Place of Presentation: New London (USA)
  • Year and Date: 2006-07-24
• [Presentation] Stability and plasticity of dendritic spines. (2005)
  • Author(s): H. Kasai
  • Organizer: Gordon Research Conference on Excitatory Amino Acids.
  • Place of Presentation: Ausoir (France)
  • Year and Date: 2005-09-13
• [Presentation] Dendritic spine structures critical for spine functions and plasticity. (2005)
  • Author(s): H. Kasai
  • Organizer: Cold Spring Harbor Symposium on Learning and Memory.
  • Place of Presentation: Cold Spring Harbor (NY, USA)
  • Year and Date: 2005-04-21
• [Presentation] Two-photon excitation imaging of exocytosis and endocytosis. (2004)
  • Author(s): H. Kasai
  • Organizer: Gordon Research Conference on Cell Biology of the Neuron.
  • Place of Presentation: New London (NH, USA)
  • Year and Date: 2004-06-23
• [Remarks] 新聞発表
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  • Industrial Property Rights Holder: 河西春郎、松崎政紀、オリンパス株式会社
  • Industrial Property Number: 特願2007-285938
  • Filing Date: 2007-01-12
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  • Inventor(s): 岸本拓哉、河西春郎
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Number: 特願2005-107593
  • Filing Date: 2005-07-07